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Surgical Sperm Retrieval (PESA and TESA)
Surgical sperm retrieval is a technique for collecting sperm from the vas deferens, epididymis or testis. This is a minor procedure, usually carried as a day case surgery under a local or a general anesthetic.


There are selected groups of patients to whom surgical sperm retrieval is recommended.

Men with complete absence of sperm in their ejaculate when the sperm production by the testicles is normal but there is a blockage, which prevents sperm entering the semen (obstructive azoospermia). This may be due to a blockage of sperm transport tubules (epididymis or vas deferens) for whatever reasons, congenital absence of the vas deference, a previous vasectomy or failed vasectomy reversal.

Men with complete absence of sperm from their ejaculate due to failure of sperm production by the testicles, about 50% of these men may have areas in their testicles producing sperm, which do not pass into semen although there is no obstruction (non-obstructive azoospermia). Testicular biopsy from these men may have some sperm which can be used for intracytoplasmic sperm injection (ICSI).

There are two methods of retrieving sperm surgically.

micro tese

PESA (percutaneous sperm aspiration)

This is the first choice (if possible) to collect sperm. A fine needle is inserted through the scrotum into the epididymis and sperm are obtained by gentle suction. After each sample is collected, it is examined under the microscope to confirm the presence of sperm.

TESA (testicular sperm extraction)

testicular extract

A fine needle is inserted into the testis and sample of tissue are obtained by gentle suction and examined under the microscope. If sperm are not found, a small tissue sample (testicular biopsy) is taken through a small incision in the scrotum and testis, sperm can then be extracted from the tissue. The cut is stitched back together with a couple of stitches, which self dissolve in about 10 days. Finding sperm in the testicular tissue can be a laborious process. Surgically retrieved sperm are immature and incapable of fertilization by conventional means. Fertilization is achieved using ICSI, this involve injecting a selected sperm into the cytoplasm of a mature egg.

After the operation, you may feel discomfort, bruising and tenderness of the scrotum for 24-48 hours. This will be relieved with painkillers such as paracetemol or codeine tablets. There is a small risk of infection and bleeding after the procedure. A firm scrotal support is recommended until the discomfort subsides. The long-term effects of repeated testicular biopsy are poorly understood.
Any spare sperm or testicular tissues may be frozen for later use, thus avoiding a repeat of surgical sperm retrieval procedure.


Authored by Dr. Anitha Mani - Infertility Specialist & Laparoscopy Surgeon